ecl western blotting kit Search Results


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GE Healthcare chemiluminescence ecl western blotting detection kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Chemiluminescence Ecl Western Blotting Detection Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TransGen biotech co ecl western blotting detection kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Ecl Western Blotting Detection Kit, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Applygen Technologies secondary antibody conjugated to hrp and the ecl western blotting kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Secondary Antibody Conjugated To Hrp And The Ecl Western Blotting Kit, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amresco ecl western blotting substrate kit
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Ecl Western Blotting Substrate Kit, supplied by Amresco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nacalai ecl western blotting substrate 07880
Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with <t>a</t> <t>monoclonal</t> mouse antibody, clone 303.9 (ARP), and <t>ECL</t> (Amersham Pharmacia).
Ecl Western Blotting Substrate 07880, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Babco Inc ecl western blot kit
In vivo tumor targeting by systemically delivered TfRscFv/LipA-HoKC/DNA. Athymic nude mice carrying DU145 subcutaneous xenograft tumors were i.v. injected with various complexes. The animals were injected either once or three times within 24 h. Twenty-four hours after the last or single injection the tumors, liver and lung were excised and protein isolated for western analysis. An aliquot of 40 µg protein/lane was loaded. The GFP protein band was detected using <t>a</t> <t>monoclonal</t> anti-GFP antibody (BAbCO) and an <t>ECL</t> western blot kit. The same membrane was subsequently probed with an anti-GAPDH antibody to establish equal protein loading per lane.
Ecl Western Blot Kit, supplied by Babco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Applygen Technologies western ecl blotting kit ecl
In vivo tumor targeting by systemically delivered TfRscFv/LipA-HoKC/DNA. Athymic nude mice carrying DU145 subcutaneous xenograft tumors were i.v. injected with various complexes. The animals were injected either once or three times within 24 h. Twenty-four hours after the last or single injection the tumors, liver and lung were excised and protein isolated for western analysis. An aliquot of 40 µg protein/lane was loaded. The GFP protein band was detected using <t>a</t> <t>monoclonal</t> anti-GFP antibody (BAbCO) and an <t>ECL</t> western blot kit. The same membrane was subsequently probed with an anti-GAPDH antibody to establish equal protein loading per lane.
Western Ecl Blotting Kit Ecl, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beijing Solarbio Science enhanced chemiluminescence reagent
In vivo tumor targeting by systemically delivered TfRscFv/LipA-HoKC/DNA. Athymic nude mice carrying DU145 subcutaneous xenograft tumors were i.v. injected with various complexes. The animals were injected either once or three times within 24 h. Twenty-four hours after the last or single injection the tumors, liver and lung were excised and protein isolated for western analysis. An aliquot of 40 µg protein/lane was loaded. The GFP protein band was detected using <t>a</t> <t>monoclonal</t> anti-GFP antibody (BAbCO) and an <t>ECL</t> western blot kit. The same membrane was subsequently probed with an anti-GAPDH antibody to establish equal protein loading per lane.
Enhanced Chemiluminescence Reagent, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SignaGen lumigold ecl detection kit
In vivo tumor targeting by systemically delivered TfRscFv/LipA-HoKC/DNA. Athymic nude mice carrying DU145 subcutaneous xenograft tumors were i.v. injected with various complexes. The animals were injected either once or three times within 24 h. Twenty-four hours after the last or single injection the tumors, liver and lung were excised and protein isolated for western analysis. An aliquot of 40 µg protein/lane was loaded. The GFP protein band was detected using <t>a</t> <t>monoclonal</t> anti-GFP antibody (BAbCO) and an <t>ECL</t> western blot kit. The same membrane was subsequently probed with an anti-GAPDH antibody to establish equal protein loading per lane.
Lumigold Ecl Detection Kit, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Epizyme Inc ecl western blot detection system
In vivo tumor targeting by systemically delivered TfRscFv/LipA-HoKC/DNA. Athymic nude mice carrying DU145 subcutaneous xenograft tumors were i.v. injected with various complexes. The animals were injected either once or three times within 24 h. Twenty-four hours after the last or single injection the tumors, liver and lung were excised and protein isolated for western analysis. An aliquot of 40 µg protein/lane was loaded. The GFP protein band was detected using <t>a</t> <t>monoclonal</t> anti-GFP antibody (BAbCO) and an <t>ECL</t> western blot kit. The same membrane was subsequently probed with an anti-GAPDH antibody to establish equal protein loading per lane.
Ecl Western Blot Detection System, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Servicebio Inc ecl western blotting substrate kit
In vivo tumor targeting by systemically delivered TfRscFv/LipA-HoKC/DNA. Athymic nude mice carrying DU145 subcutaneous xenograft tumors were i.v. injected with various complexes. The animals were injected either once or three times within 24 h. Twenty-four hours after the last or single injection the tumors, liver and lung were excised and protein isolated for western analysis. An aliquot of 40 µg protein/lane was loaded. The GFP protein band was detected using <t>a</t> <t>monoclonal</t> anti-GFP antibody (BAbCO) and an <t>ECL</t> western blot kit. The same membrane was subsequently probed with an anti-GAPDH antibody to establish equal protein loading per lane.
Ecl Western Blotting Substrate Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amersham Life Sciences Inc ecl advance blocking agent
In vivo tumor targeting by systemically delivered TfRscFv/LipA-HoKC/DNA. Athymic nude mice carrying DU145 subcutaneous xenograft tumors were i.v. injected with various complexes. The animals were injected either once or three times within 24 h. Twenty-four hours after the last or single injection the tumors, liver and lung were excised and protein isolated for western analysis. An aliquot of 40 µg protein/lane was loaded. The GFP protein band was detected using <t>a</t> <t>monoclonal</t> anti-GFP antibody (BAbCO) and an <t>ECL</t> western blot kit. The same membrane was subsequently probed with an anti-GAPDH antibody to establish equal protein loading per lane.
Ecl Advance Blocking Agent, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with a monoclonal mouse antibody, clone 303.9 (ARP), and ECL (Amersham Pharmacia).

Journal:

Article Title: Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5- rep Sequence in cis

doi: 10.1128/JVI.76.11.5411-5421.2002

Figure Lengend Snippet: Expression of Rep from Ad-Rep210 virus. Protein was extracted from HeLa cells infected with Ad-Rep210 virus at an MOI of 10 or 30 (lanes 1 and 2, respectively), with Ad5-GFP (lane 3), and with wtAAV at an MOI of 10 (lane 4); also shown is mock extract plus 200 ng of purified bacterially expressed Rep 78 (lane 5). Extracts were separated on a sodium dodecyl sulfate-8% polyacrylamide gel and transferred to a nitrocellulose membrane as described in Materials and Methods. AAV Rep 78 protein was detected with a monoclonal mouse antibody, clone 303.9 (ARP), and ECL (Amersham Pharmacia).

Article Snippet: AAV Rep 78 protein was detected with a monoclonal mouse antibody supernatant, clone 303.9 (ARP), and an enhanced chemiluminescence (ECL) Western blotting detection kit (Amersham Pharmacia), according to the manufacturer's instructions.

Techniques: Expressing, Infection, Purification

In vivo tumor targeting by systemically delivered TfRscFv/LipA-HoKC/DNA. Athymic nude mice carrying DU145 subcutaneous xenograft tumors were i.v. injected with various complexes. The animals were injected either once or three times within 24 h. Twenty-four hours after the last or single injection the tumors, liver and lung were excised and protein isolated for western analysis. An aliquot of 40 µg protein/lane was loaded. The GFP protein band was detected using a monoclonal anti-GFP antibody (BAbCO) and an ECL western blot kit. The same membrane was subsequently probed with an anti-GAPDH antibody to establish equal protein loading per lane.

Journal:

Article Title: Enhanced transfection efficiency of a systemically delivered tumor-targeting immunolipoplex by inclusion of a pH-sensitive histidylated oligolysine peptide

doi: 10.1093/nar/gnh049

Figure Lengend Snippet: In vivo tumor targeting by systemically delivered TfRscFv/LipA-HoKC/DNA. Athymic nude mice carrying DU145 subcutaneous xenograft tumors were i.v. injected with various complexes. The animals were injected either once or three times within 24 h. Twenty-four hours after the last or single injection the tumors, liver and lung were excised and protein isolated for western analysis. An aliquot of 40 µg protein/lane was loaded. The GFP protein band was detected using a monoclonal anti-GFP antibody (BAbCO) and an ECL western blot kit. The same membrane was subsequently probed with an anti-GAPDH antibody to establish equal protein loading per lane.

Article Snippet: The GFP protein band was detected using a monoclonal anti-GFP antibody (BAbCO) and an ECL western blot kit.

Techniques: In Vivo, Injection, Isolation, Western Blot